{"id":3610,"date":"2020-09-07T10:35:07","date_gmt":"2020-09-07T10:35:07","guid":{"rendered":"https:\/\/www.editage.com\/insights\/lab-on-paper-strip-small-inexpensive-platform-for-diagnosing-tropical-fevers\/"},"modified":"2025-01-15T06:29:45","modified_gmt":"2025-01-15T06:29:45","slug":"lab-on-paper-strip-small-inexpensive-platform-for-diagnosing-tropical-fevers","status":"publish","type":"post","link":"https:\/\/www.editage.com\/insights\/lab-on-paper-strip-small-inexpensive-platform-for-diagnosing-tropical-fevers","title":{"rendered":"Lab-on-paper strip: Small, inexpensive platform for diagnosing tropical fevers"},"content":{"rendered":"<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">The ongoing COVID-19 pandemic has shown how important it is to have tools that can rapidly diagnose viral infectious diseases. Aside from the SARS-CoV-2 virus, which has currently taken the spotlight, the zika, dengue, and chikungunya viruses have also become major threats to human health in many parts of the world, particularly in tropical countries. All three viruses are transmitted by mosquitoes and cause diseases that have similar symptoms, making early diagnosis particularly difficult without complex molecular diagnostic equipment. <\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">In a recent effort to make the diagnosis of these mosquito-borne diseases faster and easier, a team of scientists, led by Professor Min-Gon Kim from the Gwangju Institute of Science and Technology in Korea, have developed a compact, fully automatic, and inexpensive tool that can identify the presence of these viruses from a blood serum sample. The device, named <i>LAMDA <\/i>(<\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">stands for <i>lab-on-paper for all-in-one molecular diagnostics<\/i><\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">)<i> <\/i>by the scientists<\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">, is essentially a mini laboratory on a paper strip<\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">\u2014vaguely <\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">reminiscent of over-the-counter pregnancy tests. <\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">LAMDA performs all the steps of a standard nucleic acid test (a molecular diagnostic test), namely sampling, extraction, amplification, and detection of the target viral RNA, without external intervention at any intermediate step. To use LAMDA, one has to simply place a drop of blood serum and some drops of distilled water on two pads. The liquids naturally flow through the paper strip horizontally and reach the base of a small vertical stack of layers that extracts all the RNA from the sample and multiplies any existing viral RNA of the three diseases. <\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">The top layer of the vertical stack comprises individual \u201creaction\u201d patches, each designed to detect one of the three diseases. After the RNA is extracted, it flows up to the top layer, where \u201cLAMP (Loop-mediated isothermal amplification)\u201d reactions cause the fluorescent indicators on a patch to become dim if its target viral RNA is present in the sample. <\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">In this way, LAMDA can correctly diagnose any of the three mosquito-borne diseases in less than an hour. Excited about the results, which are <span class=\"MsoHyperlink\" style=\"color:blue\"><span style=\"text-decoration:underline\"><a href=\"https:\/\/doi.org\/10.1016\/j.bios.2020.112400\" style=\"color:blue; text-decoration:underline\">published in Elsevier\u2019s <i>Biosensors and Bioelectronics<\/i><\/a><\/span><\/span>, Prof Kim remarks: \u201c<i>We believe that with minor modifications, such as a portable system to maintain reaction temperature at 65<\/i><\/span><i><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">\u00b0<\/span><\/i><i><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">C and a means to detect the fluorescence change with a smartphone, the proposed all-in-one paper chip can become a portable, low-cost, user-friendly, sensitive, and specific nucleic acid test platform with great potential in point-of-care diagnostics.<\/span><\/i><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">\u201d <\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">LAMDA could be an excellent option for resource-limited clinics and hospitals, which are unfortunately common among countries most affected by mosquito-borne diseases. It could also bolster future research in the field of diagnostics for other infectious diseases. \u201c<i>We certainly hope that our approach and achievements with LAMDA will be helpful to advance research and development of on-site medical diagnostic tools,<\/i>\u201d Prof Kim concludes.<\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Perhaps, a generation of accurate and high-utility <i>pocket<\/i> diagnostic tools is on the horizon!<\/span><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><strong>Reference<\/strong><br \/>\u00a0<\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"tab-stops:83.1pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">A<\/span><\/span><\/b><\/span><\/span><\/span><span style=\"font-size:12pt\"><span style=\"tab-stops:83.1pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">uthors<\/span><\/span><\/b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">: <\/span><\/span><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Youngung Seok (1), Bhagwan S. Batule (2,3), and Min-Gon Kim (2*)<\/span><\/span><\/span><\/span><\/span><br \/>\u00a0<\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Title of original paper<\/span><\/span><\/b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">: Lab-on-paper for all-in-one molecular diagnostics (LAMDA) of zika, dengue, and chikungunya virus from human serum<\/span><\/span><\/span><\/span><br \/>\u00a0<\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"tab-stops:83.1pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Journal<\/span><\/span><\/b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">: <i>Biosensors and Bioelectronics<\/i><\/span><\/span><\/span><\/span><\/span><br \/>\u00a0<\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"tab-stops:83.1pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">DOI<\/span><\/span><\/b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">: <\/span><\/span><span class=\"MsoHyperlink\" style=\"color:blue\"><span style=\"text-decoration:underline\"><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\"><a href=\"https:\/\/doi.org\/10.1016\/j.bios.2020.112400\" style=\"color:blue; text-decoration:underline\">10.1016\/j.bios.2020.112400<\/a><\/span><\/span><\/span><\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Affiliations<\/span><\/span><\/b><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">1. Department of Mechanical Engineering and Applied Mechanics, School of Engineering and Applied Science, University of Pennsylvania <\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">2. Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST) <\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-size:11.0pt\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">3. Boditech Med Inc.<\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">*Corresponding author\u2019s email: <span class=\"MsoHyperlink\" style=\"color:blue\"><span style=\"text-decoration:underline\"><a href=\"mailto:mkim@gist.ac.kr\" style=\"color:blue; text-decoration:underline\">mkim@gist.ac.kr<\/a><\/span><\/span><\/span><\/span><\/span><\/p>\n<p style=\"margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p class=\"Default\" style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:Calibri,sans-serif\"><span style=\"color:black\"><b>About <\/b><b>Gwangju Institute of Science and Technology (GIST) <\/b><\/span><\/span><\/span><\/p>\n<p class=\"Default\" style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\"><a href=\"http:\/\/www.gist.ac.kr\/\">Gwangju Institute of Science and Technology<\/a> (GIST) is a research-oriented university situated in Gwangju, South Korea. One of the most prestigious schools in South Korea, it was founded in 1993.<span style=\"background:white\"> The university aims to create a strong research environment to spur advancements in science and technology and to promote collaboration between foreign and domestic research programs<\/span>. With its motto, \u201cA Proud Creator of Future Science and Technology,\u201d the university has consistently received one of the highest university rankings in Korea. <\/span><\/span><\/span><\/span><\/p>\n<p class=\"Default\" style=\"margin:0in 0in 0.0001pt\">\u00a0<\/p>\n<p class=\"Default\" style=\"margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"font-family:Calibri,sans-serif\"><span style=\"color:black\"><b>About the author<\/b><\/span><\/span><\/span><\/p>\n<p style=\"text-align:justify; margin:0in 0in 0.0001pt\"><span style=\"font-size:12pt\"><span style=\"text-justify:inter-ideograph\"><span style=\"font-family:&quot;Times New Roman&quot;,serif\"><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Dr. Min-Gon Kim is a Professor of the Department of Chemistry at the <\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">Gwangju Institute of Science and Technology<\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">. His research group focuses on the development of biosensors and nanobiomaterials by the integration of bio-organic and inorganic materials. Dr. Kim\u2019s Lab is developing rapid, high-sensitivity and affordable biosensors for diagnostics and food safety. Another focus area is biomolecule immobilization and bio-nanoprobe syntheses. In particular, Kim\u2019s research group has achieved great success in the development <\/span><span style=\"font-family:&quot;Cambria&quot;,serif\">of <\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\">lab-on paper devices,<\/span><span style=\"font-family:&quot;Calibri&quot;,sans-serif\"> LSPR, colorimetric, FRET, and CRET biosensors to detect target analytes.<\/span><\/span><\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The ongoing COVID-19 pandemic has shown how important it is to have tools that can rapidly diagnose viral infectious diseases. Aside from the SARS-CoV-2 virus, which has currently taken the spotlight, the zika, dengue, and chikungunya viruses have also become major threats to human health in many parts of the world, particularly in tropical countries. [&hellip;]<\/p>\n","protected":false},"author":1152,"featured_media":33313,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[2435],"tags":[2482],"new_categories":[],"new_tags":[],"series":[],"class_list":["post-3610","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-trending-research","tag-science-update"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Lab-on-paper strip: Small, inexpensive platform for diagnosing tropical fevers | Editage Insights<\/title>\n<meta name=\"description\" content=\"Small, inexpensive platform for diagnosing tropical fevers developed by researchers from Gwangju Institute of Science and Technology in Korea that can readily detect the specific RNA of these viruses from a small blood 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